Journal: Frontiers in Endocrinology

Article Title: Anoctamin 5 mutation leads to abnormal bone homeostasis of GDD by regulating AMPK-dependent glucose metabolism

doi: 10.3389/fendo.2026.1703491

Figure Lengend Snippet: AMPK inhibitor regulates osteogenesis and osteoclastogenesis of GDD in vivo . 12-week-old male mice were used to observe bone phenotype. (A) Representative photomicrographs and quantitative immuno-positive area analysis of OCN in tibia (bar=100 μm); (B–D) ELISA analysis of serum level of ALP, PINP (B) , OPG, OPG/RANKL (C) , and CTX (D, E) Representative images of TRAP staining and quantitative analysis of the osteoclast number (Oc.N)/perimeter of bone (B.Pm) in the germinal center of the tibia (bar=100 μm). Data are presented as mean ± SEM. Statistic significances are determined by one-way ANOVAs with Dunnett’s multiple comparison tests, with ns: no significance, *P < 0.05, **P < 0.01.

Article Snippet: Procollagen I N-terminal propeptide (PINP) and type I collagen cross-linked C-telopeptide (CTX) in the serum were measured by using mouse PINP ELISA Kit (Elabscience, E-EL-M0233) and mouse β-CTx ELISA Kit (Elabscience, E-EL-M0372) following the manufacturer’s protocol.

Techniques: In Vivo, Enzyme-linked Immunosorbent Assay, Staining, Comparison

Journal: Advanced Science

Article Title: Epigenetic Targeting of Senescent Cells Prevents the Deleterious Effects of Obstructive Sleep Apnea on Growing Skeleton

doi: 10.1002/advs.202502697

Figure Lengend Snippet: CIH induces bone loss and growth retardation in young mice. Three‐week‐old male or female wild‐type (WT) mice were subjected to CIH or normoxia condition for four weeks. A–E) Representative micro‐CT images of both male and female mice were shown in (A) with quantitative analysis of BV/TV (B), Tb. N (C), Tb. Th (D) and Tb. Sp (E). F,G) The serum concentrations of P1NP and CTX of both male and female mice were measured by ELISA. General view of the femurs was shown in H), and the length of the femurs were measured in I). J,K) H&E staining was performed to show the general morphology of the femoral bone and growth plate. RZ, resting zone; PZ, proliferating zone; HZ, hypertrophic zone; GP, growth plate. The fold change of GP length, RZ length, PZ length, and HZ length was quantified in L–O). n = 5‐6 mice Data were represented as mean ± SD. p values were determined by one‐way ANOVA.

Article Snippet: N‐terminal propeptide of type I procollagen (P1NP) was measured using P1NP ELISA kit (E‐EL‐M0233, Elabscience) and β‐CTx ELISA kit (E‐EL‐M0372, Elabscience) as described by the company.

Techniques: Micro-CT, Enzyme-linked Immunosorbent Assay, Staining

Journal: Advanced Science

Article Title: Epigenetic Targeting of Senescent Cells Prevents the Deleterious Effects of Obstructive Sleep Apnea on Growing Skeleton

doi: 10.1002/advs.202502697

Figure Lengend Snippet: Elevated H3K27me3 alleviates CIH‐induced osteoprogenitor senescence and bone loss. Three‐week‐old female UTX iKO mice and WT (UTX floxed) mice were subjected to CIH or normoxia condition for four weeks. A,B) Representative co‐immunofluorescence staining of OSX and H3K27me3 in distal femur section in (A) and analysis of cell number per mm 2 tissue area (N. OSX + H3K27me3 + cells/ Ar) in (B). C,D) Representative images of SA‐βGal staining in the distal femur section in (C) and analysis of cell number per mm 2 tissue area (N. SA‐βGal + cells/ Ar) in (D). E,F) Representative co‐immunofluorescence staining of OSX and γ H2A.X in the distal femur section in (E) and analysis of cell number per mm 2 tissue area (N. OSX + γ H2A.X + cells/ Ar) in (F). G,H) Representative immunofluorescence staining of OCN in distal femur section in (G) and analysis of cell number per mm 2 tissue area (N. OCN + cells/ Ar) in (H). Representative micro‐CT images were shown in I) with quantitative analysis of BV/TV J), Tb. N K), Tb. Th L) and Tb. Sp M). N,O) The concentrations of P1NP and CTX were measured by ELISA. n = 5‐6 mice. Data were represented as mean ± SD. p ‐values were determined by one‐way ANOVA.

Article Snippet: N‐terminal propeptide of type I procollagen (P1NP) was measured using P1NP ELISA kit (E‐EL‐M0233, Elabscience) and β‐CTx ELISA kit (E‐EL‐M0372, Elabscience) as described by the company.

Techniques: Immunofluorescence, Staining, Micro-CT, Enzyme-linked Immunosorbent Assay

Journal: Advanced Science

Article Title: Epigenetic Targeting of Senescent Cells Prevents the Deleterious Effects of Obstructive Sleep Apnea on Growing Skeleton

doi: 10.1002/advs.202502697

Figure Lengend Snippet: GSK‐J4 rescues CIH‐impaired bone growth and mineral acquisition. Three‐week‐old female WT mice were subjected to CIH or normoxia condition for four weeks, and at the same time, treated with GSK‐J4 at 10 mg kg −1 per day or vehicle. Representative micro‐CT images were shown in A) with quantitative analysis of BV/TV B), Tb. N C), Tb. Th D) and Tb. Sp E). F,G) The concentrations of P1NP and CTX were measured by ELISA. General view of the femurs was shown in H), and the length of the femurs were measured in I). J,K) H&E staining was performed to show tissue morphology. RZ, resting zone; PZ, proliferating zone; HZ, hypertrophic zone; GP, growth plate. The fold change of GP length, RZ length, PZ length, and HZ length were quantified in L–O). n = 5–6 mice. Data were represented as mean ± SD. p ‐values were determined by one‐way ANOVA.

Article Snippet: N‐terminal propeptide of type I procollagen (P1NP) was measured using P1NP ELISA kit (E‐EL‐M0233, Elabscience) and β‐CTx ELISA kit (E‐EL‐M0372, Elabscience) as described by the company.

Techniques: Micro-CT, Enzyme-linked Immunosorbent Assay, Staining